Home / IL-17F / Mouse IL-17F DuoSet ELISA

Mouse IL-17F DuoSet ELISA

Catalog #: DY2057
13 Citations Datasheet / COA / SDS

Discontinued Product

DY2057 has been discontinued.
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Mouse IL-17F ELISA Standard Curve
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Citations (13)
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse IL-17F DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
23.4 - 1,500 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-17F. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Mouse IL-17F ELISA Standard Curve View Larger

Mouse IL-17F ELISA Standard Curve

Product Datasheets

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Product Datasheet
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SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17F

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Long Name:
Interleukin 17F
Entrez Gene IDs:
112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)
Alternate Names:
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; interleukin 17F

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Mouse IL-17F DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Ablation of RhoA impairs Th17 cell differentiation and alleviates house dust mite-triggered allergic airway inflammation
    Authors: JQ Yang, KW Kalim, Y Li, Y Zheng, F Guo
    J. Leukoc. Biol., 2019-07-01;0(0):.
    Species: Mouse
    Sample Types: BALF
  2. Suppression of IL-17F, but not of IL-17A, provides protection against colitis by inducing Treg cells through modification of the intestinal microbiota
    Authors: C Tang, S Kakuta, K Shimizu, M Kadoki, T Kamiya, T Shimazu, S Kubo, S Saijo, H Ishigame, S Nakae, Y Iwakura
    Nat. Immunol., 2018-06-18;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Th17 cells are refractory to senescence and retain robust antitumor activity after long-term ex vivo expansion
    Authors: JS Bowers, MH Nelson, K Majchrzak, SR Bailey, B Rohrer, AD Kaiser, C Atkinson, L Gattinoni, CM Paulos
    JCI Insight, 2017-03-09;2(5):e90772.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. A critical role for the TLR signaling adapter Mal in alveolar macrophage-mediated protection against Bordetella pertussis.
    Authors: Bernard N, Finlay C, Tannahill G, Cassidy J, O'Neill L, Mills K
    Mucosal Immunol, 2014-12-17;8(5):982-92.
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Allergic airway inflammation decreases lung bacterial burden following acute Klebsiella pneumoniae infection in a neutrophil- and CCL8-dependent manner.
    Authors: Dulek D, Newcomb D, Goleniewska K, Cephus J, Zhou W, Reiss S, Toki S, Ye F, Zaynagetdinov R, Sherrill T, Blackwell T, Moore M, Boyd K, Kolls J, Peebles R
    Infect Immun, 2014-06-23;82(9):3723-39.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. Subcomponent vaccine based on CTA1-DD adjuvant with incorporated UreB class II peptides stimulates protective Helicobacter pylori immunity.
    Authors: Nedrud J, Bagheri N, Schon K, Xin W, Bergroth H, Eliasson D, Lycke N
    PLoS ONE, 2013-12-31;8(12):e83321.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Interleukin-23-mediated inflammation in Pseudomonas aeruginosa pulmonary infection.
    Authors: Dubin PJ, Martz A, Eisenstatt JR, Fox MD, Logar A, Kolls JK
    Infect. Immun., 2011-10-24;80(1):398-409.
    Species: Mouse
    Sample Types: BALF
  8. IL-1RL2 and its ligands contribute to the cytokine network in psoriasis.
    Authors: Blumberg H, Dinh H, Dean C, Trueblood ES, Bailey K, Shows D, Bhagavathula N, Aslam MN, Varani J, Towne JE, Sims JE
    J. Immunol., 2010-09-10;185(7):4354-62.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. IL-23 is required for protection against systemic infection with Listeria monocytogenes.
    Authors: Meeks KD, Sieve AN, Kolls JK, Ghilardi N, Berg RE
    J. Immunol., 2009-12-15;183(12):8026-34.
    Species: Mouse
    Sample Types: Serum
  10. TLR2 deficiency leads to increased Th17 infiltrates in experimental brain abscesses.
    Authors: Nichols JR, Aldrich AL, Mariani MM, Vidlak D, Esen N, Kielian T
    J. Immunol., 2009-06-01;182(11):7119-30.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses.
    Authors: Ishigame H, Kakuta S, Nagai T, Kadoki M, Nambu A, Komiyama Y, Fujikado N, Tanahashi Y, Akitsu A, Kotaki H, Sudo K, Nakae S, Sasakawa C, Iwakura Y
    Immunity, 2009-01-16;30(1):108-19.
    Species: Mouse
    Sample Types: Whole Tissue
  12. Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
    Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
    J. Immunol., 2008-09-15;181(6):4299-310.
    Species: Mouse
    Sample Types: BALF
  13. IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner.
    Authors: Wei L, Laurence A, Elias KM, O'Shea JJ
    J. Biol. Chem., 2007-09-20;282(48):34605-10.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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