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Mouse Pentraxin 3/TSG-14 DuoSet ELISA

Catalog #: DY2166
8 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY2166
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse Pentraxin 3 / TSG-14 ELISA Standard Curve
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Product Details
Procedure
Citations (8)
FAQs
Supplemental Products
Reviews (1)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse Pentraxin 3/TSG-14 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 minutes (after plate preparation)
Sample Volume Required
100 µL
Assay Range
218.0 - 14,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Pentraxin 3 (PTX3/TSG-14). The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Mouse Pentraxin 3 / TSG-14 ELISA Standard Curve View Larger

Mouse Pentraxin 3 / TSG-14 ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

Long Name:
Tumor Necrosis Factor Stimulated Gene 14
Entrez Gene IDs:
5806 (Human); 19288 (Mouse)
Alternate Names:
alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Mouse Pentraxin 3/TSG-14 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Long pentraxin PTX3 is upregulated systemically and centrally after experimental neurotrauma, but its depletion leaves unaltered sensorimotor deficits or histopathology
    Authors: M Oggioni, D Mercurio, D Minuta, S Fumagalli, K Popiolek-B, M Sironi, A Ciechanows, S Ippati, D De Blasio, C Perego, J Mika, C Garlanda, MG De Simoni
    Scientific Reports, 2021-05-05;11(1):9616.
    Species: Mouse
    Sample Types: Plasma
  2. Role of a fluid-phase PRR in fighting an intracellular pathogen: PTX3 in Shigella infection
    Authors: V Ciancarell, L Lembo-Fazi, I Paciello, AK Bruno, S Jaillon, S Berardi, M Barbagallo, S Meron-Suda, D Cohen, A Molinaro, G Rossi, C Garlanda, ML Bernardini
    PLoS Pathog., 2018-12-07;14(12):e1007469.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. The Long Pentraxin 3 Plays a Role in Bone Turnover and Repair
    Authors: D Gr?evi?, M Sironi, S Valentino, L Deban, H Cvija, A Inforzato, N Kova?i?, V Katavi?, T Kelava, I Kalajzi?, A Mantovani, B Bottazzi
    Front Immunol, 2018-03-05;9(0):417.
    Species: Mouse
    Sample Types: Serum
  4. Vaccination with a live attenuated Acinetobacter baumannii deficient in thioredoxin provides protection against systemic Acinetobacter infection
    Authors: S Ainsworth, PM Ketter, JJ Yu, RC Grimm, HC May, AP Cap, JP Chambers, MN Guentzel, BP Arulananda
    Vaccine, 2017-05-15;0(0):.
    Species: Mouse
    Sample Types: Serum
  5. Role of pentraxin 3 in shaping arthritogenic alphaviral disease: from enhanced viral replication to immunomodulation.
    Authors: Foo, Suan-Sin, Chen, Weiqiang, Taylor, Adam, Sheng, Kuo-Chin, Yu, Xing, Teng, Terk-Shi, Reading, Patrick, Blanchard, Helen, Garlanda, Cecilia, Mantovani, Alberto, Ng, Lisa F P, Herrero, Lara J, Mahalingam, Suresh
    PLoS Pathog, 2015-02-19;11(2):e1004649.
    Species: Mouse
    Sample Types: Serum
  6. Severe Acinetobacter baumannii sepsis is associated with elevation of pentraxin 3.
    Authors: Ketter P, Guentzel M, Schaffer B, Herzig M, Wu X, Montgomery R, Parida B, Fedyk C, Yu J, Jorgensen J, Chambers J, Cap A, Arulanandam B
    Infect Immun, 2014-07-07;82(9):3910-8.
    Species: Mouse
    Sample Types: Serum
  7. Exogenous pentraxin 3 restores antifungal resistance and restrains inflammation in murine chronic granulomatous disease.
    Authors: D'Angelo C, D'Angelo C, De Luca A, Zelante T, Bonifazi P, Moretti S, Giovannini G, Iannitti RG, Zagarella S, Bozza S, Campo S, Salvatori G, Romani L
    J. Immunol., 2009-09-04;183(7):4609-18.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. The long pentraxin PTX3 is crucial for tissue inflammation after intestinal ischemia and reperfusion in mice.
    Authors: Souza DG, Amaral FA, Fagundes CT, Coelho FM, Arantes RM, Sousa LP, Matzuk MM, Garlanda C, Mantovani A, Dias AA, Teixeira MM
    Am. J. Pathol., 2009-03-12;174(4):1309-18.
    Species: Mouse
    Sample Types: Serum

FAQs

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Reviews for Mouse Pentraxin 3/TSG-14 DuoSet ELISA

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Mouse Pentraxin 3/TSG-14 DuoSet ELISA
By Anonymous on 03/11/2022
Sample Tested: Serum and Plasma

works very well for mouse serum samples