This protocol outlines a method for the culture of organoid progenitor cells from normal and healthy mouse gastric, small intestine, or colon tissues using Cultrex® Organoid Qualified Basement Membrane Extract as a scaffold. Some specific tips and considerations you can find in this protocol include:
- Considerations and tips for starting enteric organoid cultures from frozen stock
- How Cultrex Organoid Qualified BME performs as a scaffold to support enteric mouse organoid cultures
- Maintenance of mouse enteric organoid cultures, including tips for successful passaging
- Cryobanking and preservation of enteric organoids for analysis and future experiments
View All Resources and Reagents for Organoid Research
