This protocol outlines a method for the culture of normal human gastric organoids using Cultrex® Organoid Qualified Basement Membrane Extracts (BME) as a scaffold. Some specific tips and considerations you can find in this protocol include:
- Considerations and tips for starting gastric organoid cultures
- How Cultrex Organoid Qualified BME performs as a scaffold to support organoid cultures
- Maintenance of gastric organoid cultures, including tips for successful passaging
- Cryobanking and preservation of organoids for analysis and future experiments
