This protocol outlines a method for preparation of embedding organoids in Cultrex® Basement Membrane Extract (BME) and further analysis of the organoids by immunofluorescence. Some specific tips and considerations you can find in this protocol include:
- Methods and tips for organoid culture, harvesting, and processing
- Use of Cultrex BME for the conservation of organoid structures and other 3-D culture types
- Challenges when immunostaining embedded organoids
- Tips and considerations to improve organoid preservation and immunostaining when embedding in Cultrex BME
View All Resources and Reagents for Organoid Research
