This protocol outlines a method for the harvesting of organoids from culture as well as the processing recommendations for passaging and biochemical analysis using the Cultrex® Organoid Harvesting Solution. Some specific tips and considerations you can find in this protocol include:
- Method for the non-enzymatic depolymerization of extracellular matrix proteins for passaging and analysis of organoid cultures
- Tips and considerations to improve organoid harvest for protein expression analysis by Western blot or qPCR
- Organoid culture and differentiation considerations for mouse small intestine organoids
